zldmЗаводская лаборатория. Диагностика материаловIndustrial laboratory. Diagnostics of materials1028-68612588-0187ООО «Издательство «ТЕСТ-ЗЛ»zldm-139Research ArticleАНАЛИЗ ВЕЩЕСТВАSUBSTANCES ANALYSISИдентификация и определение микотоксинов и пестицидов в кормах из одной навески методом высокоэффективной жидкостной хроматографии с времяпролетной масс-спектрометрией высокого разрешенияIdentification and Determination of Mycotoxins and Pesticides in Feed from Single Sample by HPLC/TOF High Resolution Mass SpectrometryАмелинВ. Г.AmelinV. G.amelinvg@mail.ruАндораловА. М.AndoralovA. M.noemail@neicon.ruТимофеевА. А.TimofeevA. A.noemail@neicon.ruФедеральный центр охраны здоровья животных, г. Владимир, Россия; Владимирский государственный университет им. А. Г. и Н. Г. Столетовых, г. Владимир, РоссияRussian FederationВладимирский государственный университет им. А. Г. и Н. Г. Столетовых, г. Владимир, Россия; Брянская межобластная ветеринарная лаборатория, г. Брянск, РоссияRussian Federation20150110201581101423Copyright © Амелин В.Г., Андоралов А.М., Тимофеев А.А., 20152015Амелин В.Г., Андоралов А.М., Тимофеев А.А.Amelin V.G., Andoralov A.M., Timofeev A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.zldm.ru/jour/article/view/139

Разработан способ одновременной идентификации и определения 25 микотоксинов и 170 пестицидов и их метаболитов в кормах на зерновой и растительной основе для животных и птиц из одной навески методом времяпролетной масс-спектрометрии высокого разрешения по точным массам ионов в сочетании с высокоэффективной жидкостной хроматографией и простой экспрессной пробоподготовкой. Предложена схема идентификации и определения обнаруживаемых аналитов методом стандартной добавки. Нижняя граница определяемых содержаний составляет 1(500) мкг/кг. Относительное стандартное отклонение результатов анализа не превышает 0,1. Продолжительность скрининга проб составляет 30 - 40 мин, количественного анализа - 1 - 1,5 ч.

A method for simultaneous identification and determination of 25 mycotoxins and 170 pesticides and their metabolites in grain and vegetable-based animal feed for animals and birds from single sample using time of flight high-resolution mass spectrometry combined with high-performance liquid chromatography and easy express sample preparation is developed. A scheme for identification and determination of the detected analytes by the method of standard additive is proposed. The lower limit of determination is 1(500) pg/kg. The relative standard deviation of the test results does not exceed 0.1. The duration of the sample screening and quantification is 30 - 40 min and 1 - 1.5 h, respectively.

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The authors declare that there are no conflicts of interest present.