zldmЗаводская лаборатория. Диагностика материаловIndustrial laboratory. Diagnostics of materials1028-68612588-0187ООО «Издательство «ТЕСТ-ЗЛ»10.26896/1028-6861-2022-88-12-14-20zldm-1813Research ArticleАНАЛИЗ ВЕЩЕСТВАSUBSTANCES ANALYSISMicrospherical polystyrene-divinylbenzene particles hybridized with eremomycin stabilized gold nanoparticles as a stationary phase for chiral liquid chromatographyMicrospherical polystyrene-divinylbenzene particles hybridized with eremomycin stabilized gold nanoparticles as a stationary phase for chiral liquid chromatographyProsuntsovaD. S.ProsuntsovaD. S.

Darya S. Prosuntsova - Chemistry Department.

1-3 Leninskie gory, Moscow, 119991

Darya S. Prosuntsova - Chemistry Department.

1-3 Leninskie gory, Moscow, 119991

inhusoria6@yandex.ruAnanievaI. A.AnanievaI. A.

Irina A. Ananieva - Chemistry Department.

1-3 Leninskie gory, Moscow, 119991

Irina A. Ananieva - Chemistry Department.

1-3 Leninskie gory, Moscow, 119991

NesterenkoP. N.NesterenkoP. N.

Pavel N. Nesterenko - Chemistry Department, Lomonosov Moscow State University; Research and Science Department, Shenzhen MSU-BIT University.

1-3 Leninskie gory, Moscow, 119991; 299, Ruyi Rd., Longgang district, Shenzhen, 518172, China

Pavel N. Nesterenko - Chemistry Department, Lomonosov Moscow State University; Research and Science Department, Shenzhen MSU-BIT University.

1-3 Leninskie gory, Moscow, 119991; 299, Ruyi Rd., Longgang district, Shenzhen, 518172, China

ShpigunO. A.ShpigunO. A.

Oleg A. Shpigun - Chemistry Department.

1-3 Leninskie gory, Moscow, 119991

Oleg A. Shpigun - Chemistry Department.

1-3 Leninskie gory, Moscow, 119991

Lomonosov Moscow State UniversityРоссияLomonosov Moscow State UniversityRussian FederationLomonosov Moscow State University; Shenzhen MSU-BIT UniversityРоссияLomonosov Moscow State University; Shenzhen MSU-BIT UniversityRussian Federation20222512202288121420Copyright © Prosuntsova D.S., Ananieva I.A., Nesterenko P.N., Shpigun O.A., 20222022Prosuntsova D.S., Ananieva I.A., Nesterenko P.N., Shpigun O.A.Prosuntsova D.S., Ananieva I.A., Nesterenko P.N., Shpigun O.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.zldm.ru/jour/article/view/1813

A novel enantioselective adsorbent was obtained by hybridization of microspherical polystyrene-divinylbenzene (PS-DVB) macroporous particles with eremomycin-stabilized gold nanoparticles (GNPs). Macrocyclic antibiotic eremomycin was used as a stabilization agent to obtain GNPs which were then characterized by transmission electron microscope. The average diameter of obtained nanoparticles is about 16.6 nm. Eremomycin-stabilized nanoparticles were successfully embedded into the porous polymer structure with a resulting chiral selector content of 37.5 pmol/g. The obtained PS-DVB composite containing GNPs with immobilized eremomycin was studied by scanning electron microscopy and diffuse reflectance spectroscopy. The values of the specific surface area (500 m2/g) and porosity of the adsorbent (0.39 cm3/g) are measured using nitrogen adsorption at low temperatures. The obtained composite material was used as a chiral stationary phase of liquid chromatography. A good separation enantio-selectivity to amino acids, their derivatives and beta-blockers under RPC (reversed-phase) and HILIC (Hidrophilic Interaction Liquid Chromatography) mode is demonstrated. The results obtained revealed that the prepared Eremo@GNP@PS-DVB composite is promising for use as a stationary phase in HPLC.

A novel enantioselective adsorbent was obtained by hybridization of microspherical polystyrene-divinylbenzene (PS-DVB) macroporous particles with eremomycin-stabilized gold nanoparticles (GNPs). Macrocyclic antibiotic eremomycin was used as a stabilization agent to obtain GNPs which were then characterized by transmission electron microscope. The average diameter of obtained nanoparticles is about 16.6 nm. Eremomycin-stabilized nanoparticles were successfully embedded into the porous polymer structure with a resulting chiral selector content of 37.5 pmol/g. The obtained PS-DVB composite containing GNPs with immobilized eremomycin was studied by scanning electron microscopy and diffuse reflectance spectroscopy. The values of the specific surface area (500 m2/g) and porosity of the adsorbent (0.39 cm3/g) are measured using nitrogen adsorption at low temperatures. The obtained composite material was used as a chiral stationary phase of liquid chromatography. A good separation enantio-selectivity to amino acids, their derivatives and beta-blockers under RPC (reversed-phase) and HILIC (Hidrophilic Interaction Liquid Chromatography) mode is demonstrated. The results obtained revealed that the prepared Eremo@GNP@PS-DVB composite is promising for use as a stationary phase in HPLC.

poly(styrene-divinylbenzene)gold nanoparticlesliquid chromatographychiral stationary phasemacrocyclic antibioticpoly(styrene-divinylbenzene)gold nanoparticlesliquid chromatographychiral stationary phasemacrocyclic antibioticThis work was supported by the Russian Foundation for Fundamental Research (Grant N 20-33-90177). The authors are thankful to Dr. S.M. Staroverov (BioChemmack ST, Moscow, Russia) for providing extra pure samples of eremomycine. This work was supported in part by Lomonosov Moscow State University Program of Development by providing Perkin Elmer 2400 Series II CHNS/O Elemental Analyzer.This work was supported by the Russian Foundation for Fundamental Research (Grant N 20-33-90177). The authors are thankful to Dr. S.M. Staroverov (BioChemmack ST, Moscow, Russia) for providing extra pure samples of eremomycine. 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The authors declare that there are no conflicts of interest present.