zldmЗаводская лаборатория. Диагностика материаловIndustrial laboratory. Diagnostics of materials1028-68612588-0187ООО «Издательство «ТЕСТ-ЗЛ»10.26896/1028-6861-2023-89-10-19-24zldm-2032Research ArticleАНАЛИЗ ВЕЩЕСТВАSUBSTANCES ANALYSISNovel stationary phases based on epoxidized polystyrene-divinylbenzene for three modes of liquid chromatographyNovel stationary phases based on epoxidized polystyrene-divinylbenzene for three modes of liquid chromatographyGorbovskaiaА. V.GorbovskaiaA. V.

Anastasiia V. Gorbovskaia

Department of Chemistry

1 bld. 3, Leninskie gory, Moscow, 119991

TalipovaI. I.TalipovaI. I.

Ilsina I. Talipova

Department of Chemistry

1 bld. 3, Leninskie gory, Moscow, 119991

TimichevА. А.TimichevA. A.

Arsenii A. Timichev

Department of Chemistry

1 bld. 3, Leninskie gory, Moscow, 119991

UzhelА. S.UzhelA. S.

Anna S. Uzhel

Department of Chemistry

1 bld. 3, Leninskie gory, Moscow, 119991

anna_uzhel@mail.ruShpigunO. А.ShpigunO. A.

Oleg A. Shpigun

Department of Chemistry

1 bld. 3, Leninskie gory, Moscow, 119991

Lomonosov Moscow State UniversityRussian Federation20232510202389101924Copyright © Gorbovskaia А.V., Talipova I.I., Timichev А.А., Uzhel А.S., Shpigun O.А., 20232023Gorbovskaia А.V., Talipova I.I., Timichev А.А., Uzhel А.S., Shpigun O.А.Gorbovskaia A.V., Talipova I.I., Timichev A.A., Uzhel A.S., Shpigun O.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.zldm.ru/jour/article/view/2032

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Mixed-mode stationary phases based on epoxidized copolymer of styrene and divinylbenzene have been obtained by amination with methylamine, further alkylation with 1,4-butanediol diglycidyl ether and opening the terminal oxirane rings with dimethylethanolamine. To evaluate the effect of the number of anchor amino and diol groups on the degree of hydrophilization of resins, the quantity of reagents was varied. Polymerization of glycidol in the functional layer at an increased pH of the reaction medium was carried out for additional shielding of the substrate. It was found that increasing the number of anchor amino groups is promising for increasing hydrophilicity in suppressed ion chromatography and hydrophilic interaction liquid chromatography modes, while polymerization of glycidol increases the degree of substrate shielding. The applicability of the most hydrophilic adsorbent in three HPLC modes was demonstrated. Novel mixed-mode stationary phase allows the separation of six nucleosides and nitrogenous bases by hydrophilic interaction liquid chromatography, 7 alkylbenzenes by reversed phase liquid chromatography, and 20 organic and inorganic anions by suppressed ion chromatography.

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The authors declare that there are no conflicts of interest present.