zldmЗаводская лаборатория. Диагностика материаловIndustrial laboratory. Diagnostics of materials1028-68612588-0187ООО «Издательство «ТЕСТ-ЗЛ»10.26896/1028-6861-2025-91-8-16-22zldm-2571Research ArticleАНАЛИЗ ВЕЩЕСТВАSUBSTANCES ANALYSISОпределение содержания жирных кислот с сопряженными C=C связями в масле гранатовых косточек методом спектроскопии комбинационного рассеяния светаDetermination of fatty acids with conjugated C=C bonds in pomegranate seed oil using Raman spectroscopyКузнецовС. М.KuznetsovS. M.

Сергей Михайлович Кузнецов

119991, Москва, ул. Вавилова, д. 38

Sergey M. Kuznetsov

38, ul. Vavilova, Moscow, 119991

kuznetsovsm@kapella.gpi.ruНовиковВ. С.NovikovV. S.

Василий Сергеевич Новиков

119991, Москва, ул. Вавилова, д. 38

Vasiliy S. Novikov

38, ul. Vavilova, Moscow, 119991

ЛаптинскаяП. К.LaptinskayaP. K.

Полина Константиновна Лаптинская

119991, Москва, ул. Вавилова, д. 38

Polina K. Laptinskaya

38, ul. Vavilova, Moscow, 119991

КудрявцевО. С.KudryavtsevO. S.

Олег Сергеевич Кудрявцев

119991, Москва, ул. Вавилова, д. 38

Oleg S. Kudryavtsev

38, ul. Vavilova, Moscow, 119991

МосковскийМ. Н.MoskovskiyM. N.

Максим Николаевич Московский

109428, Москва, 1-й Институтский пр., д. 5

Maksim N. Moskovskiy

5, 1-y Institutsky proezd, Moscow, 109428

СагитоваЕ. А.SagitovaE. A.

Елена Александровна Сагитова

119991, Москва, ул. Вавилова, д. 38

Elena A. Sagitova

38, ul. Vavilova, Moscow, 119991

Институт общей физики им. А. М. Прохорова Российской академии наукРоссияProkhorov General Physics Institute of the Russian Academy of SciencesRussian FederationФедеральный научный агроинженерный центр ВИМРоссияFederal Scientific Agroengineering Center VIMRussian FederationProkhorov General Physics Institute of the Russian Academy of SciencesRussian Federation2025310820259181622Copyright © Кузнецов С.М., Новиков В.С., Лаптинская П.К., Кудрявцев О.С., Московский М.Н., Сагитова Е.А., 20252025Кузнецов С.М., Новиков В.С., Лаптинская П.К., Кудрявцев О.С., Московский М.Н., Сагитова Е.А.Kuznetsov S.M., Novikov V.S., Laptinskaya P.K., Kudryavtsev O.S., Moskovskiy M.N., Sagitova E.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.zldm.ru/jour/article/view/2571

Впервые предложен подход для быстрой оценки содержания пуниковой кислоты в масле гранатовых косточек по спектрам комбинационного рассеяния (КР) света. С использованием двух длин волн возбуждающего излучения (532 и 785 нм) исследовали спектры КР смесей масла гранатовых косточек и подсолнечного масла. Смеси этих масел рассматривали в качестве модельных систем масла гранатовых косточек с различным содержанием пуниковой кислоты. С привлечением теории функционала плотности рассчитан спектр КР молекулы пуниковой кислоты. На основании этих исследований установлено, что отношение пиковых интенсивностей I1628/I1442 спектральных линий с частотами 1628 и 1442 см–1 пропорционально содержанию пуниковой кислоты в масле. Линия КР около 1628 см–1 связана с валентными колебаниями C=C связей в последовательностях из трех сопряженных связей в молекулах этой кислоты. Линия около 1442 см–1 относится к деформационным колебаниям C–H связей в CH2-группах в молекулах всех жирных кислот в масле гранатовых косточек. Показано, что метод спектроскопии КР позволяет идентифицировать пуниковую кислоту в масле гранатовых косточек даже при низких содержаниях (1 % масс.). Дополнительно обнаружено, что спектры КР, зарегистрированные с использованием длины волны возбуждения 532 нм, позволяют детектировать наличие каротиноидов в масле гранатовых косточек.

For the first time, we suggest a Raman spectroscopic method for rapid evaluation of punicic acid content in pomegranate seed oil. In this contribution, we study Raman spectra of mixtures of pomegranate seed oil and sunflower oil as model systems of pomegranate seed oil with different punicic acid content using two excitation wavelengths (532 and 785 nm). The spectrum of punicic acid, calculated using the density functional theory, is also considered. We found that the ratio I1628/I1442 of the peak intensities of Raman lines at 1628 and 1442 cm–1 is proportional to the punicic acid content. The line at 1628 cm–1 is related to the stretching vibrations of double carbon-carbon bonds (C=C) in the sequence of three conjugated bonds in punicic acid molecule. The line at 1442 cm–1 belongs to the scissoring C–H vibrations in CH2 groups in molecules of all fatty acids of pomegranate seed oil. We revealed that Raman spectroscopy permits detecting punicic acid even at very low contents (up to 1 wt.%). In addition, we found that the record-ing Raman spectra using excitation wavelength of 532 allows one to detect carotenoids in pomegranate seed oil.

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The authors declare that there are no conflicts of interest present.