zldmЗаводская лаборатория. Диагностика материаловIndustrial laboratory. Diagnostics of materials1028-68612588-0187ООО «Издательство «ТЕСТ-ЗЛ»10.26896/1028-6861-2025-91-11-26-33zldm-2654Research ArticleАНАЛИЗ ВЕЩЕСТВАSUBSTANCES ANALYSISОпределение полиароматических углеводородов в почвах методом высокоэффективной жидкостной хроматографии с использованием техники QuEChERSHigh-performance liquid chromatography determination of polycyclic aromatic hydrocarbons in soils using the QuEChERS techniqueОвсепянС. К.OvsepyanS. K.

Сюзанна Кареновна Овсепян 

350040, г. Краснодар, ул. Ставропольская, д. 149

Suzanna K. Ovsepyan 

149, Stavropol’skaya ul., Krasnodar, 350040

ТемердашевЗ. А.TemerdashevZ. A.

Зауаль Ахлоович Темердашев

350040, г. Краснодар, ул. Ставропольская, д. 149

Zaual A. Temerdashev 

149, Stavropol’skaya ul., Krasnodar, 350040

temza@kubsu.ruЦюпкоТ. Г.TsyupkoT. G.

Татьяна Григорьевна Цюпко

350040, г. Краснодар, ул. Ставропольская, д. 149

Tatyana G. Tsyupko

149, Stavropol’skaya ul., Krasnodar, 350040

Кубанский государственный университетРоссияKuban State UniversityRussian Federation20252611202591112633Copyright © Овсепян С.К., Темердашев З.А., Цюпко Т.Г., 20252025Овсепян С.К., Темердашев З.А., Цюпко Т.Г.Ovsepyan S.K., Temerdashev Z.A., Tsyupko T.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.zldm.ru/jour/article/view/2654

Разработана методика определения 18 полициклических ароматических углеводородов (ПАУ) в почвах и донных отложениях, сочетающая технику QuEChERS извлечения аналитов с последующим определением методом ВЭЖХ с флуориметрическим и диодно-матричным детектированием. Пределы определения аналитов составили 0,015 – 1,0 мкг/кг при степенях извлечения 80 – 100 %. Относительные стандартные отклонения повторяемости и воспроизводимости методики определения ПАУ изменялись в пределах 1,5 – 10 и 2,3 – 12 % соответственно. Метрологические характеристики предложенной методики сравнили с соответствующими значениями для определения ПАУ методом газовой хромато-масс-спектрометрии (ГХ-МС) также в сочетании с техникой QuEChERS: пределы определения ряда ПАУ удалось снизить на два порядка величины. Оптимизированная схема определения ПАУ в почвах апробирована при анализе образцов песка, отобранных на побережье Черного моря после аварийного затопления танкеров с мазутом: были идентифицированы ПАУ различной молекулярной массы, среди легких преобладали 2-метилнафталин, фенантрен, флуорантен и пирен; тяжелых — трифенилен, хризен и бенз[b]флуорантен. Суммарное содержание ПАУ в образцах песка составило не более 10 мкг/кг, что близко к уровню фонового загрязнения.

A method for the determination of 18 polycyclic aromatic hydrocarbons (PAHs) in soils and sediments has been developed, combining the QuEChERS technique of analyte extraction with subsequent determination by HPLC with fluorescence and diode array detection (FLD/DAD). The limits of HPLC-FLD/DAD quantification of analytes were established, which amounted to 0.015 – 1.0 μg/kg while recoveries were 80 – 100%. The relative standard deviations of the repeatability and reproducibility of the PAHs determination method ranged from 1.5 to 10 and from 2.3 to 12%, respectively. The metrological characteristics of the proposed approach were compared with the corresponding values for the PAHs determination by gas chromatography – mass spectrometry also in combination with the QuEChERS technique: the quantification limits of a number of PAHs have been reduced by two orders of magnitude. An optimized scheme for the determination of PAHs in soils has been tested in the analysis sand samples collected on the Black Sea coast after the emergency flooding of fuel oil tankers. PAHs of various molecular weights were identified: 2-methylnaphthalene, phenanthrene, fluoranthene, pyrene were predominant among the light PAHs, while the heavy PAHs were primarily represented by triphenylene, chrysene, and benzo[b]fluoranthene. The total content of certain PAHs in the sand samples did not exceed 10 μg/kg, close to the level of background pollution.

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The authors declare that there are no conflicts of interest present.