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Determination of levamisole using ion-selective electrode
https://doi.org/10.26896/1028-6861-2021-87-3-20-23
Abstract
Current development of applied ionometry requires focusing of theoretical studies both on revealing the nature of the selectivity of electrode membranes and on the developing the new methods of membrane synthesis and their modification with the goal of obtaining more perfect structural units with improved functional properties. Elucidation of the relationship between the structural characteristics of the membranes and their effect on the electro-analytical properties is a rather important task. The interaction of organic cation levamisole with bromophenol blue was studied to meet the goals. Levamisole-selective sensor with a plasticized polyvinyl chloride (PVC) membrane has been developed. The electrode contains an ionic associate of levamisole with bromophenol blue. PVC was used as a matrix to model the membrane composition. Membranes plasticized with dibutyl phthalate (DBP), diethyl phthalate (DEP), dioctyl phthalate (DOP), dinonyl phthalate (DNP), dibutyl sebacate (DBP), tricresyl phosphate (TCP) were studied. It was shown that the nature of the plasticizer insignificantly affected the analytical characteristics of the electrode. The response is linear within the concentration range of 1 × 10–5 – 1 ×10–1 mole/liter with the slope of the electrode function 52.1 ± 1.0 mV/pC. The sensor has a quick response (10 sec) and can be used for at least 8 weeks without any divergence in the long run. The operation life of the membranes with a high plasticizer content is much longer. The electrode can be used within pH range of 3.0 – 8.0. Potentiometric selectivity coefficients of the electrode relative to a number of potentially interfering ions were determined. The developed electrode was tested in determination of levamisole in model solutions and pharmaceuticals («Decaris» and «Levamisole-Zdorovye»).
About the Authors
N. V. Zubenia
Rivne Medical Academy
Ukraine
Ukraine
Natalia V. Zubenia
53, Mykoly Karnaukhova St., Rivne, 33000
Z. A. Kormosh
Eastern European National University
Ukraine
Ukraine
Zholt A. Kormosh
13, Voli av., Lutsk, 43025
I. M. Khmeliar
Rivne Medical Academy
Ukraine
Ukraine
Inesa M. Khmeliar
53, Mykoly Karnaukhova St., Rivne, 33000
O. V. Sadovnyk
Rivne Medical Academy
Ukraine
Ukraine
Olga V. Sadovnyk
53, Mykoly Karnaukhova St., Rivne, 33000
References
1. Mashkovsky M. D. Medicines. — Moscow: Meditsina, 1986. — 624 p. [in Russian].
2. Farber H. A. Clinical use of levamisole perspectives and warnings / Terap. Arkh. 1980. N I. P. 95 – 100 [in Russian].
3. Kovalev I. E. Levamisole as an immunostimulant / Khim.-Farm. Zh. 1980. N 4. P. 115 – 121 [in Russian].
4. Adams J. G. Pharmacokinetics of levamisole / J. Rheumatol. 1978. Vol. 5. P. 137 – 142.
5. International Pharmacopoeia. Vol. 2. Specifications for quality control of pharmaceuticals. — Geneva: World Health Organization, 1983. — 364 p. [in Russian].
6. Woestenborghs R., Michielsen L., Heykants J. Determination of levamisole in plasma and animal tissues by gas chromatography with thermionic specific detection / J. Chromatogr. B. 1981. Vol. 224. P. 25 – 32. DOI: 10.1016/s0378-4347(00)80134-6
7. Kouassi E., Caille G., Lery L., et al. Novel assay and pharmacokinetics of levamisole and p-hydroxylevamisole in human plasma and urine / Biopharm. Drug Dispos. 1986. Vol. 7. N 1. P. 71 – 89. DOI: 10.1002/bdd.2510070110
8. Jing X., Kai S., Wei-Feng D. Determination of levamisole residue in animal livers by two liquid liquid extraction steps-gas chromatography-mass spectrometry / Chin. J. Chromatogr. 2012. Vol. 30. N 9. P. 922 – 925. DOI: 10.3724/SP.J.1123.2012.04030
9. De Baere S., Cherlet M., Croubles S., et al. Liquid Chromatographic determination of levamisole in animal plasma: ultraviolet versus tandem mass specrometric detection / Anal. Chim. Acta. 2003. Vol. 483. P. 215 – 224. DOI: 10.1016/S0003-2670(02)01377-6
10. Jedziniak P., Szprengier-Juszkiewicz T., Olejnik M. Determination of benzimidazoles and levamisole residues in milk by liquid chromatography — mass spectrometry: Screening method development and validation / J. Chromatogr. A. 2009. Vol. 1216. N 46. P. 8165 – 8172. DOI: 10.1016/j.chroma.2009.07.036
11. Tong L., Ding L., Li Y., et al. A sensitive LC — MS/MS method for determination of levamisole in human plasma: Application to pharmacokinetic study / J. Chromatogr. B. 2011. Vol. 879. N 5 – 6. P. 299 – 303. DOI: 10.1016/j.jchromb.2010.11.015
12. Cherlet M., De Baere S., Croubels S., De Backer P. Quantitative analysis of levamisole in porcine tissues by high-performance liquid chromatography combined with atmospheric pressure chemical ionization mass spectrometry / J. Chromatogr. B. 2000. Vol. 742. N 2. P. 283 – 293. DOI: 10.1016/S0378-4347(00)00171-7
13. Alvinerie M., Galtier P., Escoular G. Ion-pair high-performance liquid chromatographic assay of levamisole in biological fluids / J. Chromatogr. B. 1981. Vol. 223. P. 445 – 448. DOI: 10.1016/s0378-4347(00)80120-6
14. El-Kholy H., Kemppamen B. W. Liquid chromatographic method with ultraviolet absorbance detection for measurement of levamisole in chicken tissues, eggs and plasma / J. Chromatogr. B. 2003. Vol. 796. P. 371 – 377. DOI: 10.1016/j.jchromb.2003.08.025
15. Vandamme Th. F., Demoustier M., Rollmann B. Quantitation of levamisole in plasma using high performance liquid chromatography / Eur. J. Drug Metab. Pharmacokinet. 1995. Vol. 20. N 2. P. 145 – 149. DOI: 10.1007/BF03226369
16. Sowjanya S., Devadasu Ch. Development of RP-HPLC Method for the Simultaneous Quantitation of Levamisole and Albendazole: Application to Assay Validation / Int. J. Anal. Chem. 2018. Vol. 2018:5746305. DOI: 10.1155/2018/5746305
17. Marriner S., Galbraith E. A., Bogan J. A. Determination of the anthelmintic levamisole in plasma and gastrointestinal fuids by high-performance liquid chromatography / Analyst. 1980. Vol. 105. N 1255. P. 993 – 996. DOI: 10.1039/AN9800500993
18. Garcia J. J., Diez M. J., Sierra M., Teran M. T. Determination of Levamisole by HPLC in Plasma Samples in the Presence of Heparin and Pentobarbital / J. Liq. Chromatogr. 1990. Vol. 13. N 4. P. 743 – 749. DOI: 10.1080/01483919008051817
19. Holbrook A., Scales B. Polarographic determination of tetramisole hydrochloride in extracts of animal tissue / Anal. Biochem. 1967. Vol. 18. P. 46 – 53. DOI: 10.1016/0003- 2697(67)90054-1
20. Calatayud J. M., Falco C. Determination of levamisole hydrochloride with by a turbidimetric method and flow-injection analysis / Talanta. 1986. Vol. 33. N 8. P. 685 – 687. DOI: 10.1016/0039-9140(86)80158-8
21. Sadeghi S., Fathi F., Abbasivar J. Potentiometric sensing of levamisole hydrochloride based on moleculary imprinted polymer / Sens. Actuat. B. 2007. Vol. 122. N 1. P. 158 – 164. DOI: 10.1016/j.snb.2006.05.018
22. Kamman K. Working with ion-selective electrodes. — Moscow: Mir, 1972. — 283 p. [Russian translation].
23. Morf W. E. The Principles of Ion-Selective Electrodes and of Membrane Transport. — Moscow: Mir, 1985. — 280 p. [Russian translation].
24. Zubenya N., Kormosh Zh. Potentiometric membrane sensor for levamisole determination / Mediterr. J. Chem. 2017. Vol. 6. N 2. P. 7 – 14. DOI: 10.13171/mjc61/016111516/kormosh
Review
For citations:
Zubenia N.V., Kormosh Z.A., Khmeliar I.M., Sadovnyk O.V. Determination of levamisole using ion-selective electrode. Industrial laboratory. Diagnostics of materials. 2021;87(3):20-23. (In Russ.) https://doi.org/10.26896/1028-6861-2021-87-3-20-23
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