Определение метилфосфоновой кислоты и ее алкиловых эфиров в почве методом жидкостной хромато-масс-спектрометрии высокого разрешения

Оптимизирован способ одновременного извлечения из почвы этилового эфира метилфосфоновой кислоты (ЭМФК), изопропилового эфира метилфосфоновой кислоты (ИПМФК), изобутилового эфира метилфосфоновой кислоты (ИБМФК), пинаколилового эфира метилфосфоновой кислоты (ПМФК) и метилфосфоновой кислоты (МФК) — высокополярных продуктов гидролиза нервно-паралитических отравляющих веществ — с последующим определением методом ВЭЖХ — тандемной масс-спектрометрии высокого разрешения. Приведены наблюдаемые ионы в масс-спектрах фрагментации депротонированных молекул ЭМФК, ИПМФК, ИБМФК, ПМФК, МФК и их дейтерированных аналогов (d3-ЭМФК, d3-ИПМФК, d3-ИБМФК, d3-ПМФК и d3-МФК), а также возможные структурные формулы фрагментных ионов. В качестве способа подготовки проб почвы к анализу выбрали водную экстракцию деионизированной водой с последующим концентрированием путем упаривания. Разделение компонентов проводили в варианте обращенно-фазовой хроматографии. Время, необходимое для выполнения пробоподготовки и анализа проб почвы, не превышает 1 ч. Пределы обнаружения в почве составили: 0,05 нг/г ЭМФК и ИПМФК, 0,02 нг/г ИБМФК и ПМФК, 1 нг/г для МФК.

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