Rapid HPLC-MS/MS Determination of Methylphosphonic Acid in Environmental Water
Abstract
New rapid LC-MS/MS approach to determination of methylphosphonic acid (MPA) in environmental waters is developed. MPA is a final product of nerve agent hydrolysis. Optimal conditions of LC separation and MS/MS detection are specified. The developed method is characterized by good sensitivity, precision, selectivity. Matrix component do not interfere with the determination in analysis of natural water samples. The developed approach was tested in analysis of spiked samples of environmental water. The detection limit of MPA direct LC-MS/MS determination in environmental waters is 10 ng/ml.
About the Authors
T. M. Baygildiev
Московский государственный университет им. М. В. Ломоносова
Russian Federation
Russian Federation
I. A. Rodin
Московский государственный университет им. М. В. Ломоносова
Russian Federation
Russian Federation
A. N. Stavrianidi
Московский государственный университет им. М. В. Ломоносова; Костромской государственный технологический университет
Russian Federation
Russian Federation
A. V. Braun
Московский государственный университет им. М. В. Ломоносова; Костромской государственный технологический университет
Russian Federation
Russian Federation
D. I. Akhmerova
Московский государственный университет им. М. В. Ломоносова
Russian Federation
Russian Federation
O. A. Shpigun
Московский государственный университет им. М. В. Ломоносова
Russian Federation
Russian Federation
I. V. Rybalchenko
Костромской государственный технологический университет
Russian Federation
Russian Federation
References
1. Convention on the Prohibition of the Development, Production, Stockpiling and Use of Chemical Weapons and their Destruction, Technical Secretariat of the Organization for the Prohibition of Chemical Weapons, The Hague, 1997. URL: http://www.opcw.nl.
2. Mill T., Gould C. W. Free-radical oxidation of organic phosphonic acid salts in water using hydrogen peroxide, oxygen, and ultraviolet light / Environ. Sci. Technol. 1979. Vol. 13. P. 205 - 208.
3. Daughton C. G., Cook A. M., Alexander M. Phosphate and soil binding: factors limiting bacterial degradation of ionic phosphorus-containing pesticide metabolites / Appl. Environ. Microbiol. 1979. Vol. 37. N 3. P. 605 -609.
4. Schowanek D., Verstraete W. Phosphonate utilization by bacterial cultures and enrichments from environmental samples / Appl. Environ. Microbiol. 1990. Vol. 56. N 4. P. 895 - 903.
5. Osamura N., Murata K., Kimura A. Cytosolic C-P Bond Cleavage Activity in Bacterial Cells Isolated from Soil / J. Ferment. Bioeng. 1991. Vol. 71. N2. P. 128- 130.
6. Lyman W. J., Reehl W. F., Rosenblatt D. H. Handbook of Chemical Property Estimation Methods. - Washington, DC: American Chemical Society, 1990. - 54 p.
7. Mercier J.-P., Morin Ph., Dreux M., Tambute A. Capillary electrophoresis analysis of chemical warfare agent breakdown products I. Counterelectroosmotic separation of alkylphosphonic acids and their monoester derivatives / J. Chromatogr. A. 1996. Vol. 741. P. 279 - 285.
8. Mercier J.-P., Morin Ph., Dreux M., Tambute A. Capillary electrophoresis separation of alkylphosphonic acid monoesters with indirect ultraviolet detection / J. Chromatogr. A. 1997. Vol. 779. P. 245 - 252.
9. Oehrle S. A., Bossle P. C. Analysis of nerve agent degradation products using capillary ion electrophoresis / J. Chromatogr. A. 1995. Vol. 692. P. 247-252.
10. Rohrbaugh D. K., Sarver E. W. Detection of alkyl methylphosphonic acids in complex matrices by gas chromatography-tandem mass spectrometry / J. Chromatogr. A. 1998. Vol. 809. P. 141 - 150.
11. Sega G. A., Tomkins B. A., Griest W. H. Analysis of methylphosphonic acid, ethyl methylphosphonic acid and isopropyl methylphosphonic acid at low microgram per liter levels in groundwater / J. Chromatogr. A. 1997. Vol. 790. P. 143 - 152.
12. Katagi M., Nishikawa M., Tatsuno M., Tsuchihash H. Determination of the main hydrolysis product of O-ethyl S-2-diisopropylaminoethyl methylphosphonothiolate, ethyl methylphosphonic acid, in human serum / J. Chromatogr. B. 1997. Vol. 689. P. 327 - 333.
13. Tornes J. Aa., Johnsen B. A. Gas chromatographic determination of methylphosphonic acids by methylation with trimethylphenylammonium hydroxide / J. Chromatogr. A. 1989. Vol. 467. P. 129 - 138.
14. Pardasani D., Palit M., Gupta A., Kanaujia P., Sekhar K., Dubey D. Microemulsion mediated in situ derivatization-extraction and gas chromatography-mass spectrometric analysis of alkylphosphonic acids / J. Chromatogr. A. 2006. Vol. 1108. P. 166 - 175.
15. Saradhi V., Prabhakar S., Redy T. J., Vairamani M. Ion-pair solidphase extraction and gas chromatography-mass spectrometric determination of acidic hydrolysis products of chemical warfare agents from aqueous samples / J. Chromatogr. A. 2006. Vol. 1129. P. 9 - 13.
16. Pianetti G. A., Moreira de Campos L. M. Application of ion chromatography with indirect spectrophotometric detection to the sensitive determination of alkylphosphonic acids and fosfomycin / Anal. Chim. Acta. 1993. Vol. 284. P. 291 - 299.
17. Katagi M., Nishikawa M., Tatsuno M., Tsuchihashi H. Determination of the main hydrolysis products of organophosphorus nerve agents, methylphosphonic acids, in human serum by indirect photometric detection ion chromatography / J. Chromatogr. B. 1997. Vol. 698. P. 81 - 88.
18. Creasy W. R. Postcolumn Derivatization Liquid Chromatography/Mass Spectrometry for Detection of Chemical-Weapons-Related Compounds / J. Am. Soc. Mass Spectrom. 1999. Vol. 10. P. 440 - 447.
19. Black R. M., Read R. W. Application of liquid chromatography-atmospheric pressure chemical ionisation mass spectrometry, and tandem mass spectrometry, to the analysis and identification of degradation products of chemical warfare agents / J. Chromatogr. A. 1997. Vol. 759. P. 79 - 92.
20. Black R. M., Read R. W. Analysis of degradation products of organophosphorus chemical warfare agents and related compounds by liquid chromatography-mass spectrometry using electrospray and atmospheric pressure chemical ionization / J. Chromatogr. A. 1998. Vol. 794. P. 233 -244.
21. Hayes T. L., Kenny D. V., Hernon-Kenny L. Feasibility of direct analysis of saliva and urine for phosphonic acids and thiodiglycol-related species associated with exposure to chemical warfare agents using LC-MS/MS / J. Med. Chem. Def. 2004. Vol. 2. N 1. P. 121 - 127.
Review
For citations:
Baygildiev T.M., Rodin I.A., Stavrianidi A.N., Braun A.V., Akhmerova D.I., Shpigun O.A., Rybalchenko I.V. Rapid HPLC-MS/MS Determination of Methylphosphonic Acid in Environmental Water. Industrial laboratory. Diagnostics of materials. 2016;82(2):5-8. (In Russ.)
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