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Taguchi method-based optimization of key parameters for the preparation of poly(lactic-co-glycolic acid) microspheres to enhance betamethasone acetate loading
https://doi.org/10.26896/1028-6861-2025-91-9-19-27
Abstract
This study presents an investigation into the effects of key process parameters on the encapsulation of betamethasone acetate (BA) in poly(lactic-co-glycolic acid) (PLGA) microspheres. The Taguchi method was employed to identify the most influential factors affecting microsphere characteristics. The experimental design included variation of several parameters: PLGA concentration, BA concentration, the aqueous-to-organic phase ratio and stirring speed. The results indicate that BA loading is primarily influenced by its own concentration and the polymer concentration. High-performance liquid chromatography was used for determination of betamethasone acetate. Optimal loading was achieved at PLGA and drug concentrations of 100 and 20 mg/mL, respectively, an aqueous-to-organic phase ratio of 50:1, and a stirring speed of 500 rpm. Under these conditions, the drug loading reached 4.35%, compared to 1.44% prior to optimization. These findings may support the refinement of PLGA microsphere preparation techniques for other active pharmaceutical ingredients with similar physicochemical properties.
About the Authors
Yu. V. Ulianova
Mendeleev University of Chemical Technology
Russian Federation
Russian Federation
Yulia V. Ulianova
9, Miusskaya pl., Moscow, 125047
E. G. Vinokurov
Mendeleev University of Chemical Technology ; Frumkin Institute of Physical Chemistry and Electrochemistry of the RAS ; All-Russian Institute for Scientific and Technical Information of the RAS
Russian Federation
Russian Federation
Evgeny G. Vinokurov
9, Miusskaya pl., Moscow, 125047
31, Leninsky prosp., Moscow, 119071
20, ul. Usievicha, Moscow, 125190
Yu. V. Ermolenko
Mendeleev University of Chemical Technology
Russian Federation
Russian Federation
Yulia V. Ermolenko
9, Miusskaya pl., Moscow, 125047
A. N. Morozov
Mendeleev University of Chemical Technology
Russian Federation
Russian Federation
Aleksandr N. Morozov
9, Miusskaya pl., Moscow, 125047
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Review
For citations:
Ulianova Yu.V., Vinokurov E.G., Ermolenko Yu.V., Morozov A.N. Taguchi method-based optimization of key parameters for the preparation of poly(lactic-co-glycolic acid) microspheres to enhance betamethasone acetate loading. Industrial laboratory. Diagnostics of materials. 2025;91(9):19-27. (In Russ.) https://doi.org/10.26896/1028-6861-2025-91-9-19-27
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