Preview

Industrial laboratory. Diagnostics of materials

Advanced search

Voltammetric Determination of Cholesterol in Food

Abstract

We report on a cholesterol electrochemical behavior on chemically modified carbon-containing electrode. All the experiments are carried out on a voltammetric workstation. The dependence of the cholesterol electrooxidation signal on pH, electrochemical concentration (potential and time) and scan rate is demonstrated. Working conditions (pH = 6.86, v = 40 mV/sec) of cholesterol determination in model media are specified. The linear dependence ofcholesterol electrooxidation current at a potential of +1.06 V on the cholesterol concentration is observed up to 50 pmol/dm3. The detection limit is 0.07 pmol/dm3. Results of comparative determination of cholesterol in food using voltammetric and spectrophotometric procedures are presented.

About the Authors

K. V. Derina
Национальный исследовательский Томский политехнический университет
Russian Federation


E. I. Korotkova
Национальный исследовательский Томский политехнический университет
Russian Federation


E. V. Dorozhko
Национальный исследовательский Томский политехнический университет
Russian Federation


O. A. Voronova
Национальный исследовательский Томский политехнический университет
Russian Federation


I. V. Chulkova
Национальный исследовательский Томский политехнический университет
Russian Federation


References

1. Rog T., Vattulainen I. Cholesterol, sphingolipids, and glycolipids: what do we know about their role in raft-like membranes? / Chem. Phys. Lipids. 2014. Vol. 184. P. 82 - 104.

2. McNamara D. J. Encyclopedia of Human Nutrition (Third Edition). - New York: Academic Press, 2013. - 652 p.

3. Roth G. A., Fihn S. D., Mokdad A. H., et al. High total serum cholesterol, medication coverage and therapeutic control: an analysis of national health examination survey data from eight countries / Bull. World Health Org. 2011. Vol. 89(2). P.92-101.

4. Kamelska A. M., Jarmolowska B., Bryl K. A simplified enzymatic method for total cholesterol determination in milk / Int. Dairy J. 2015. Vol. 50. P. 50 - 57.

5. Mikkelsen S. R., Corton E. Bioanalytical chemistry. Hoboken, New Jersey: John Wiley & Sons, Inc., 2004. - 361 p.

6. Gonçalves Albuquerque T., Oliveira M. B. P. P., Sanches-Silva A., Costa H. S. Cholesterol determination in foods: Comparison between high performance and ultra-high performance liquid chromatography / FoodChem. 2016.Vol. 193.P. 18-25.

7. Слепченко Г. Б., Мартынюк О. А., Постников П. С. и др. Новые возможности вольтамперометрического определения фармацевтических препаратов на органомодифицированных электродах / Сиб. мед. журн. 2009. Т. 24. № 2-2. С. 21 - 24.

8. Комптон Р., Бэнкс К. Постигая вольтамперометрию. - Томск: Изд. ТПУ, 2015. - 508 с.

9. Oldham K. B., Myland J. C., Bond A. M. Electrochemical Science and Technology: Fundamentals and applications. - New York: John Wiley & Sons, 2011. - 456 p.

10. Ankush Parmar, Shweta Sharma. Derivative UV-vis absorption spectra as an invigorated spectrophotometric method for spectral resolution and quantitative analysis: Theoretical aspects and analytical applications / TrAC. Trends Anal. Chem. 2016. Vol. 77. P. 44 - 53.

11. Rajeev K. Thakur, Villette C., Aubry J. M., Delaplace G. Spectro-photometric method associated with formulation scans for application ofhydrophilic-lipophilic deviation concept in food emulsions / Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2007. Vol. 301. N 1 - 3. P. 469 - 474.

12. Yi-Jae Lee, Jae-Yeong Park. Nonenzymatic free-cholesterol detection via a modified highly sensitive macroporous gold electrode with platinum nanoparticles / Biosensors and Bioelectronics. 2010. Vol. 26. N 4. P. 1353 - 1358.

13. Jinu John, Ankit Reghuwanshi, Usha K. Aravind, Aravindakumar C. T. Development and validation of a high-performance thin layer chromatography method for the determination of cholesterol concentration / J. Food Drug Anal. 2015. Vol. 23. N2.P.219- 224.

14. Yan-Zong Chen, Shih-Yao Kao, Hao-Cheng Jian, et al. Determination of cholesterol and four phytosterols in foods without derivatiza tion by gas chromatography-tandem mass spectrometry / Journal of Food and Drug Analysis. 2015. Vol. 23. N 4. P. 636 - 644.

15. Narsingh R. Nirala, Shiju Abraham, Vinod Kumar, et al. Colorimetric detection of cholesterol based on highly efficient peroxidase mimetic activity of graphene quantum dots / Sens. Actuat. B: Chemical. 2015. Vol. 218. P. 42 - 50.


Review

For citations:


Derina K.V., Korotkova E.I., Dorozhko E.V., Voronova O.A., Chulkova I.V. Voltammetric Determination of Cholesterol in Food. Industrial laboratory. Diagnostics of materials. 2016;82(11):11-16. (In Russ.)

Views: 572


ISSN 1028-6861 (Print)
ISSN 2588-0187 (Online)