Simultaneous Determination of Tributyl Phosphate and Sodium Cholate in Blood Products Using HPLC-MS
Abstract
Tributyl phosphate and sodium cholate are used in the processing of blood products and their content is controlled in finished formulations. An easy to use procedure for sample preparation and simultaneous direct determination of residual amounts of tributyl phosphate (TBP) and sodium cholate (SC) in blood products is developed using reversed-phase HPLC with MS detection. Though sample preparation consists only in protein precipitation; the method provides SC and TBP determination in a concentration range up to 12.5 and 0.35 mg/liter with a relative error less than 11 and 7%, respectively.
About the Authors
V. V. Khasanov
Национальный исследовательский Томский государственный университет
Russian Federation
Russian Federation
K. A. Dychko
Национальный исследовательский Томский государственный университет
Russian Federation
Russian Federation
T. T. Kuryaeva
Национальный исследовательский Томский государственный университет
Russian Federation
Russian Federation
V. V. Khasanov
Национальный исследовательский Томский политехнический университет
Russian Federation
Russian Federation
References
1. Зубкова Н. В. Биотехнологические аспекты вирусной безопасности препаратов иммуноглобулинов: методология, производство, стандартизация. Автореф. дисс.. докт. фарм. наук. - Пермь, 2012. - 47 с.
2. Lin Y., Zhou J., Bi D., Chen P., Wang X., Liang S. Sodium-deoxycholate-assisted tryptic digestion and identification of proteolytically resistant proteins /Anal. Biochem. 2008. Vol. 377. P. 259 - 266.
3. Sharma K. R. Review on bile acid analysis / Int. J. Pharm. Biomed. Sci. 2012. Vol. 3(2). P. 28 - 34.
4. Nacher-Mestre J., Serrano R., Portoles T., Hernandez F. Investigation of organophosphate esters in fresh water, salt and brine samples by GC-TOF MS / Anal. Methods. 2011. Vol. 3. P. 1779 - 1785.
5. Ревельский А. И. Методология анализа объектов различного происхождения методами газовой хроматографии-масс-спектрометрии и элементного анализа на содержание следов среднелетучих органических веществ. Автореф. дисс.. докт. хим. наук. - М., 2012.
6. Танюхина О. Н., Густылева Л. К., Корягина Н. Л. и др. Исследование морской воды с применением классических методов растворной химии и метода газовой хроматографии - масс-спектрометрии / Тезис доклада «Аналитическая хроматография и капиллярный электрофорез». - Краснодар, 2010. С. 210.
7. Tributyl Phosphate: Method 5034, Issue 1. NIOSH Manual of Analytical Methods (NMAM). Fourth Edition, 1994. http://www.cdc.gov/niosh/docs/2003-154/pdfs/5034.pdf.
8. Nuber R., Maucher H., Stange E. F. Size exclusion chromatography for extraction of serum bile acids / J. Lipid Res. 1990. Vol. 31. P. 1517 - 1522.
9. Locket P. L., Gallacher D. D. An improved procedure for bile acid extraction and purification and tissue distribution in the rat / Lipids. 1989. Vol. 24. N 3. P. 221 - 223.
10. Yamaga N., Ogura Y., Yamada K., et al. Internal Standard Compounds for Quantitative Determination of Bile Acids by Gas Chromatography / Yonago Acta medica. 2001. Vol. 44. P. 91 - 105.
11. Perwaiz S., Tuchweber B., Mignault D., et al. Determination of bile acids in biological fluids by liquid chromatography-electrospray tandem mass spectrometry / J. Lipid Res. 2001. Vol. 42. N 1. P. 114 - 119.
12. Mathapati S., Galla S., Sankaranarayanan K., et al. Qualitative and quantitative detection of sodium deoxycholic acid in decellularized tissue / Indian J. Thorac. Cardiovasc. Surg. 2010. Vol. 26. P. 129 - 131.
13. ICH Q2B (R1). Validation of Analytical Procedure: Methodology. International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use. 1996. P. 11 - 12. http://www.ich.org/fileadmin/ Public_Web_Site/ICH_Products/Guidelines/Quality/Q2_R1/ Step4/Q2_R1_Guideline.pdf.
Review
For citations:
Khasanov V.V., Dychko K.A., Kuryaeva T.T., Khasanov V.V. Simultaneous Determination of Tributyl Phosphate and Sodium Cholate in Blood Products Using HPLC-MS. Industrial laboratory. Diagnostics of materials. 2015;81(3):19-22. (In Russ.)
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