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The impact of formic acid as a mobile phase additive on the retention of dead time markers in reversed-phase high-performance liquid chromatography
https://doi.org/10.26896/1028-6861-2025-91-5-5-9
Abstract
The effect of formic acid as a mobile phase additive on the retention time of five compounds (sodium nitrite, potassium iodide, acetone, uracil, oxalic acid) selected as dead time markers was studied in reversed-phase high-performance liquid chromatography (RP HPLC). All the studied compounds were shown to have weak retention on the surface of octadecylsilica gel under neutral conditions. However, the introduction of an acidic additive to the mobile phase leads to an increase in the retention of compounds giving an alkaline reaction. This effect was particularly strong for sodium nitrite. The lowest retention time was observed for oxalic acid, and it can be recommended as a dead time marker for the analysis of weakly polar and nonpolar compounds in neutral and acidic media.
About the Authors
O. I. Grinevich
Frumkin Institute of Physical Chemistry and Electrochemistry of the Russian Academy of Sciences
Russian Federation
Russian Federation
Oksana I. Grinevich
31-4, Leninsky prosp., Moscow, 119071
T. M. Baygildiev
Frumkin Institute of Physical Chemistry and Electrochemistry of the Russian Academy of Sciences; MIREA - Russian Technological University (Lomonosov Institute of Fine Chemical Technologies)
Russian Federation
Russian Federation
Timur M. Baygildiev
31-4, Leninsky prosp., Moscow, 119071
86, Vernadsky prosp., Moscow, 119571
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Review
For citations:
Grinevich O.I., Baygildiev T.M. The impact of formic acid as a mobile phase additive on the retention of dead time markers in reversed-phase high-performance liquid chromatography. Industrial laboratory. Diagnostics of materials. 2025;91(5):5-9. (In Russ.) https://doi.org/10.26896/1028-6861-2025-91-5-5-9
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