FLUORIMETRIC DETERMINATION OF SYNTHETIC FOOD DYES CARMOISINE AND PONCEAU 4R IN SOFT DRINKS
https://doi.org/10.26896/1028-6861-2018-84-2-23-27
Abstract
We report on a fluorimetric determination of synthetic food azo dyes Ponceau 4R (E124) and Carmoisine (azorubine, E122) in soft drinks. Considered azo dyes are one of the most common and dangerous synthetic dyes, often used in children’s drinks to give them attractive red shades. The choice of fluorimetric method of analysis is motivated by high sensitivity, selectivity and total rapidity of the procedure compared to other (spectrophotometric, electrochemical and chromatographic) known methods, thus being rather useful for determination of low content of the substances. Specified working conditions for dye determination in beverages: excitation wavelength of 330 nm and detection interval 350 – 500 nm. Synthetic dyes were analyzed on a «Fluorat-02-Panorama» (Lumex LLC., St. Petersburg) fluid analyzer. The obtained wavelength of azo dye fluorescence (420 nm) is used as a working wavelength for quantitative determination of azo dyes Ponceau 4R and Carmoisine (azorubin) in soft drinks. The reproducibility of the results obtained for two food azo dyes using fluorimetric and spectrophotometric methods of their determination in soft drinks is shown.
About the Authors
A. A. Nikolaeva
National Research Tomsk Polytechnic University
Russian Federation
Alena A. Nikolaeva
Russian Federation
Alena A. Nikolaeva
E. I. Korotkova
National Research Tomsk Polytechnic University
Russian Federation
Elena I. Korotkova
Russian Federation
Elena I. Korotkova
E. V. Bulycheva
National Research Tomsk Polytechnic University
Russian Federation
Elizabeth V. Bulycheva
Russian Federation
Elizabeth V. Bulycheva
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Review
For citations:
Nikolaeva A.A., Korotkova E.I., Bulycheva E.V. FLUORIMETRIC DETERMINATION OF SYNTHETIC FOOD DYES CARMOISINE AND PONCEAU 4R IN SOFT DRINKS. Industrial laboratory. Diagnostics of materials. 2018;84(2):23-27. (In Russ.) https://doi.org/10.26896/1028-6861-2018-84-2-23-27
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