Определение полиароматических углеводородов в почвах методом высокоэффективной жидкостной хроматографии с использованием техники QuEChERS

Разработана методика определения 18 полициклических ароматических углеводородов (ПАУ) в почвах и донных отложениях, сочетающая технику QuEChERS извлечения аналитов с последующим определением методом ВЭЖХ с флуориметрическим и диодно-матричным детектированием. Пределы определения аналитов составили 0,015 – 1,0 мкг/кг при степенях извлечения 80 – 100 %. Относительные стандартные отклонения повторяемости и воспроизводимости методики определения ПАУ изменялись в пределах 1,5 – 10 и 2,3 – 12 % соответственно. Метрологические характеристики предложенной методики сравнили с соответствующими значениями для определения ПАУ методом газовой хромато-масс-спектрометрии (ГХ-МС) также в сочетании с техникой QuEChERS: пределы определения ряда ПАУ удалось снизить на два порядка величины. Оптимизированная схема определения ПАУ в почвах апробирована при анализе образцов песка, отобранных на побережье Черного моря после аварийного затопления танкеров с мазутом: были идентифицированы ПАУ различной молекулярной массы, среди легких преобладали 2-метилнафталин, фенантрен, флуорантен и пирен; тяжелых — трифенилен, хризен и бенз[b]флуорантен. Суммарное содержание ПАУ в образцах песка составило не более 10 мкг/кг, что близко к уровню фонового загрязнения.

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