Optimization of sample preparation for the determination of bisphenol a in feed, animal products, and animal by-products by GC-MS

The recent revision of the values of the tolerated daily dose of bisphenol A (BPA), a widely used monomer in plastic production, towards their significant reduction makes it necessary to monitor the content of BPA in food products. The sensitivity of direct determination of BPA in animal products by gas chromatography-mass spectrometry, taking into account losses during sample preparation, is insufficient to monitor low analyte contents, and purification and concentration of samples are necessary. A scheme of sample preparation for the determination of BPA in animal products, animal feed and by-products is proposed. Acetonitrile was used for the extraction of BPA from meat and animal feed samples. The effectiveness of various purification methods (liquid-liquid, solid-phase and dispersion solid-phase extraction) has been studied. The best results were achieved by the use of solid-phase extraction on sorbent C18 after purification of aqueous acetonitrile extracts of samples with hexane with 10-fold concentration. The proposed approach allows the determination of BPA by GC-MS in meat, feed and manure in the range of contents from 1 to 100 μg/kg. The trueness of the results of the determination of BPA in samples of mixed feed and by-products according to the developed technique was confirmed by the recovery test, the relative standard deviation of the results does not exceed 0.11, the duration of the analysis of a series of 20 samples is 6 hours.

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