Determination of Antibiotics from Tetracycline Series in Milk Using HPLC with Post-Column Reaction and Fluorescence Detection
Abstract
Conditions of sensitive and selective chromatographyic determination of tetracycline complexes with Mg2+ in milk using HPLC with post-column reaction and fluorescence detection are specified. The fluorescence intensity of chelates is shown to be up to 1.8 times higher in microemulsions compared to that in water-acetonitrile medium. The detection limits are 5.8 and 25 ng/ml for tetracycline, oxytetracycline and doxycycline, respectively.
Keywords
About the Authors
I. D. Kargin
МГУ имени М. В. Ломоносова
Russian Federation
Russian Federation
L. S. Sokolova
МГУ имени М. В. Ломоносова
Russian Federation
Russian Federation
A. V. Pirogov
МГУ имени М. В. Ломоносова
Russian Federation
Russian Federation
O. A. Shpigun
МГУ имени М. В. Ломоносова
Russian Federation
Russian Federation
References
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Review
For citations:
Kargin I.D., Sokolova L.S., Pirogov A.V., Shpigun O.A. Determination of Antibiotics from Tetracycline Series in Milk Using HPLC with Post-Column Reaction and Fluorescence Detection. Industrial laboratory. Diagnostics of materials. 2015;81(2):5-9. (In Russ.)
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